We describe a method to efficiently separate retinal pigment epithelium (RPE) from the retina in human eyes and generate whole RPE/choroid flatmounts for histological and morphometric analyses of the RPE.
Ortolan, D., Volkov, A., Maminishkis, A., Sharma, R., Bharti, K. Efficient and Consistent Generation of Retinal Pigment Epithelium/Choroid Flatmounts from Human Eyes for Histological Analysis. J. Vis. Exp. (188), e64761, doi:10.3791/64761 (2022).