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Efficient and Consistent Generation of Retinal Pigment Epithelium/Choroid Flatmounts from Human Eyes for Histological Analysis
JoVE Journal
Biologie
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JoVE Journal Biologie
Efficient and Consistent Generation of Retinal Pigment Epithelium/Choroid Flatmounts from Human Eyes for Histological Analysis

Efficient and Consistent Generation of Retinal Pigment Epithelium/Choroid Flatmounts from Human Eyes for Histological Analysis

2,076 Views

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07:59 min

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October 28, 2022

DOI:

10.3791/64761-v

DOI:
10.3791/64761-v

•

07:59 min
October 28, 2022

•

4 Views
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1Ocular and Stem Cell Translational Research Section, National Eye Institute,National Institutes of Health (NIH)

Summary

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We describe a method to efficiently separate retinal pigment epithelium (RPE) from the retina in human eyes and generate whole RPE/choroid flatmounts for histological and morphometric analyses of the RPE.

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Cite this Article

Ortolan, D., Volkov, A., Maminishkis, A., Sharma, R., Bharti, K. Efficient and Consistent Generation of Retinal Pigment Epithelium/Choroid Flatmounts from Human Eyes for Histological Analysis. J. Vis. Exp. (188), e64761, doi:10.3791/64761 (2022).

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