Journal
/
Bio-ingénierie
/
Generation of Human Blood Vessel Organoids from Pluripotent Stem Cells
Journal JoVE
Bio-ingénierie
This content is Free Access.
Journal JoVE Bio-ingénierie
Generation of Human Blood Vessel Organoids from Pluripotent Stem Cells

Generation of Human Blood Vessel Organoids from Pluripotent Stem Cells

DOI:
10.3791/64715-v

•

09:46 min

•

January 20, 2023

•

,
1School of Biomedical Engineering,University of British Columbia, 2Life Sciences Institute,University of British Columbia, 3Department of Medical Genetics,University of British Columbia, 4Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA)

Chapitres

  • 00:04Introduction
  • 00:52Day 0: Generation of Pluripotent Aggregates from Single-Cell Suspension
  • 02:34Day 1: Mesoderm Induction of the Aggregates
  • 03:41Day 4: Vascular Induction and Priming of the Aggregates
  • 04:20Day 6: Aggregate Embedding and Vessel Sprout Induction
  • 06:02Day 11: Isolation and Maturation of the Blood Vessel Organoids (BVOs)
  • 07:25Results: Stepwise Progression of Human Blood Vessel Organoid Generation from hPSCs
  • 09:13Conclusion

Summary

Traduction automatique

Traduction automatique

This protocol describes the generation of self-organizing blood vessels from human pluripotent and induced pluripotent stem cells. These blood vessel networks exhibit an extensive and connected endothelial network surrounded by pericytes, smooth muscle actin, and a continuous basement membrane.

Tags

Blood Vessel OrganoidsPluripotent Stem CellsVasculogenesisAngiogenesisVascular DiseaseVascular PathologiesReproducibilityHigh ThroughputStem Cell LinesDiabetic VasculopathyAggregatesCell DissociationAggregation MediumSingle-cell SuspensionCell ViabilityMesoderm Media

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Development, Expansion, and In vivo Monitoring of Human NK Cells from Human Embryonic Stem Cells (hESCs) and Induced Pluripotent Stem Cells (iPSCs)

Transplantation of Induced Pluripotent Stem Cell-derived Mesoangioblast-like Myogenic Progenitors in Mouse Models of Muscle Regeneration

Efficient Generation and Editing of Feeder-free IPSCs from Human Pancreatic Cells Using the CRISPR-Cas9 System

Isolation of Adult Human Dermal Fibroblasts from Abdominal Skin and Generation of Induced Pluripotent Stem Cells Using a Non-Integrating Method

Scalable Generation of Mature Cerebellar Organoids from Human Pluripotent Stem Cells and Characterization by Immunostaining

Guided Differentiation of Mature Kidney Podocytes from Human Induced Pluripotent Stem Cells Under Chemically Defined Conditions

Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Fibroblast Derived Human Engineered Connective Tissue for Screening Applications

Microfluidic Fabrication of Core-Shell Microcapsules carrying Human Pluripotent Stem Cell Spheroids

Characterization of Blood Outgrowth Endothelial Cells (BOEC) from Porcine Peripheral Blood

Read Article