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Isolamento, attivazione ed espansione delle cellule T basate sulla flottazione da campioni di cellule mononucleate del sangue periferico umano utilizzando microbolle
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Flotation-Based T Cell Isolation, Activation, and Expansion from Human Peripheral Blood Mononuclear Cell Samples Using Microbubbles
DOI:

06:37 min

December 23, 2022

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Chapitres

  • 00:04Introduction
  • 00:29Isolation of T Cells with Microbubbles Using Positive Selection
  • 01:51Co-Stimulation (Activation) of the Positively Selected T Cells
  • 02:52Expansion of the Co-Stimulated Cells in Cell Culture Medium
  • 03:28Expansion of the T Cells (With or Without Prior Transduction)
  • 04:02Harvesting the T Cells and Flow Cytometry
  • 05:16Results: Assessment of Viable and Successfully Transduced Cells
  • 05:58Conclusion

Summary

Traduction automatique

L'obiettivo di questo studio è dimostrare la fattibilità della separazione basata sulla flottazione per isolare, attivare ed espandere le cellule T umane primarie.

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