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从健康和视网膜疾病特异性人诱导的多能干细胞中生成视网膜类器官

Journal JoVE
Biologie du développement
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Journal JoVE Biologie du développement

Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells

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从健康和视网膜疾病特异性人诱导的多能干细胞中生成视网膜类器官

2,675 Views

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09:47 min

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December 09, 2022

DOI:

10.3791/64509-v

DOI:

10.3791/64509-v

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09:47 min

December 09, 2022

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14 Views

Sudipta Mahato*^1,2, Trupti Agrawal*^1,2, Divya Pidishetty*^1,2, Savitri Maddileti, Vinay Kumar Pulimamidi³, Indumathi Mariappan

¹Centre for Ocular Regeneration, Prof. Brien Holden Eye Research Centre,LV Prasad Eye Institute, ²Manipal Academy of Higher Education, ³Schepens Eye Research Institute, Massachusetts Eye and Ear, Harvard Medical School

Tags

Retinal Organoids Human Induced Pluripotent Stem Cells Retinal Degeneration Retinal Pigment Epithelium In Vitro Models Cell-based Therapies Eye Development Inherited Retinal Diseases Differentiation Adherent Culture Suspension Culture Retinal Cups Spatial Positioning Morphological Features Cell Dissociation IPSC Culture Differentiation Induction Medium BFGF Noggin

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