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A Triple Primary Cell Culture Model of the Human Blood-Brain Barrier for Studying Ischemic Stroke In Vitro
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Journal JoVE Biochimie
A Triple Primary Cell Culture Model of the Human Blood-Brain Barrier for Studying Ischemic Stroke In Vitro

A Triple Primary Cell Culture Model of the Human Blood-Brain Barrier for Studying Ischemic Stroke In Vitro

3,365 Views

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11:06 min

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October 06, 2022

DOI:

10.3791/64469-v

DOI:
10.3791/64469-v

•

11:06 min
October 06, 2022

•

5 Views
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1Department of Biochemistry and Molecular Biology, Miller School of Medicine,University of Miami, 2Institute of Physiotherapy and Health Sciences,The Jerzy Kukuczka Academy of Physical Education

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Blood-brain BarrierIschemic StrokeIn Vitro ModelTriple Cell CulturePericytesAstrocytesCell Culture ProtocolCell DensityTrypsinizationCell CountingCell Seeding

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Fattakhov, N., Torices, S., Becker, S., Teglas, T., Naranjo, O., Toborek, M. A Triple Primary Cell Culture Model of the Human Blood-Brain Barrier for Studying Ischemic Stroke In Vitro. J. Vis. Exp. (188), e64469, doi:10.3791/64469 (2022).

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