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Biochemistry

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JoVE Journal Biochemistry
Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting
 

Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting

Article DOI: 10.3791/64179-v • 10:08 min • December 9th, 2022
December 9th, 2022 •
1, 1, 1, 1
1Institute of Biomedical and Clinical Sciences, Medical School, Faculty of Health and Life Sciences, University of Exeter, Hatherly Laboratories

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résumé
Automatic Translation

The present protocol highlights the application of western blotting technique to study the functions and activities of neuronal K-Cl co-transporter KCC2. The protocol describes the investigation of KCC2 phosphorylation at kinase regulatory sites Thr906/1007 via western blotting. Also, additional methods to confirm KCC2 activity are briefly highlighted in this text.

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Functions Activities Neuronal K-Cl Co-transporter KCC2 Western Blotting Mechanisms Regulation Kinase Molecular Size Phosphorylation Physiological Functions Protein Expression Complex Mixtures Proteins Of Interest Minute Protein Expression Essential Tool Protein Analysis Complex Systems Aberrant Tissue Function Disease Procedures Reagents Bead Bath Rat KCC2b Human Embryonic Kidney 293 Cells Centrifuge Tube Media Supernatant Aspirator Pipette Vacuum Pump
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