Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting
1,916 Views
•
10:08 min
•
December 09, 2022
DOI:
Summary
Automatically generated
The present protocol highlights the application of western blotting technique to study the functions and activities of neuronal K-Cl co-transporter KCC2. The protocol describes the investigation of KCC2 phosphorylation at kinase regulatory sites Thr906/1007 via western blotting. Also, additional methods to confirm KCC2 activity are briefly highlighted in this text.
Read Article
Cite this Article
Josiah, S. S., Meor Azlan, N. F., Oguro-Ando, A., Zhang, J. Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting. J. Vis. Exp. (190), e64179, doi:10.3791/64179 (2022).
Copy