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结核分枝杆菌 通过尺寸排阻色谱实现细胞外囊泡富集
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Immunologie et infection
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Journal JoVE Immunologie et infection
Mycobacterium tuberculosis Extracellular Vesicle Enrichment through Size Exclusion Chromatography
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结核分枝杆菌 通过尺寸排阻色谱实现细胞外囊泡富集

2,188 Views

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00:00 min

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May 19, 2022

DOI:

10.3791/63895-v

DOI:
10.3791/63895-v

•

00:00 min
May 19, 2022

•

5 Views
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1Mycobacteria Research Laboratories, Department of Microbiology Immunology & Pathology,Colorado State University, 2BioMARC, Infectious Disease Research Center,Colorado State University

Summary

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该方案描述了尺寸排阻色谱，这是一种从培养物上清液中富集 结核分枝杆菌 细胞外囊泡的简单且可重复的技术。

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Ryan, J. M., Dobos, K. M., Kruh-Garcia, N. A. Mycobacterium tuberculosis Extracellular Vesicle Enrichment through Size Exclusion Chromatography. J. Vis. Exp. (183), e63895, doi:10.3791/63895 (2022).

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