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使用冻热裂解和同量异位载体进行质谱分析的单细胞蛋白质组学制备
Journal JoVE
Biologie
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Journal JoVE Biologie
Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier
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使用冻热裂解和同量异位载体进行质谱分析的单细胞蛋白质组学制备

3,419 Views

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06:13 min

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December 09, 2022

DOI:

10.3791/63802-v

DOI:
10.3791/63802-v

•

06:13 min
December 09, 2022

•

10 Views
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1Department of Bioengineering,Northeastern University, 2Barnett Institute,Northeastern University, 3Department of Biology,Northeastern University

Summary

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在该协议中，我们描述了如何使用市售试剂和设备通过质谱 法制备 哺乳动物细胞以进行单细胞蛋白质组学分析，并提供手动和自动移液选项。

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Single-cell ProteomicsSCoPE2Mass SpectrometryFreeze-heat LysisIsobaric CarrierTrypsin DigestionTandem Mass TaggingLC-MS Analysis

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Petelski, A. A., Slavov, N., Specht, H. Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier. J. Vis. Exp. (190), e63802, doi:10.3791/63802 (2022).

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