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DNA帘法破译组蛋白组装的分子机制

Journal JoVE
Biochimie

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Journal JoVE Biochimie

Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique

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DNA帘法破译组蛋白组装的分子机制

1,507 Views

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06:32 min

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March 09, 2022

DOI:

10.3791/63501-v

DOI:

10.3791/63501-v

•

06:32 min

March 09, 2022

•

3 Views

Yujin Kang¹, Subin Bae¹, Soyeong An¹, Ja Yil Lee^1,2

¹Department of Biological Sciences,Ulsan National Institute of Science and Technology, ²Center for Genomic Integrity,Institute for Basic Science (IBS)

Summary

DNA curtain 是一种高通量单分子成像技术，为实时可视化各种蛋白质-DNA 相互作用提供了一个平台。本方案利用DNA帘技术研究了含有裂殖酵母的溴结构域AAA+ ATP酶Abo1的生物学作用和分子机制。

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