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Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique
Chapters
- 00:00Introduction
- 00:28Preparation of the Flow Cell
- 02:16Connecting Flow Cell to the Microfluidic System and Loading on Microscope
- 02:49Histone Assembly by Abo1 Using DNA Curtain
- 03:46Results: Visualization of Histone Loading by Abo1 Using Single‐Molecule DNA Curtain
- 05:46Conclusion
DNA curtain, a high-throughput single-molecule imaging technique, provides a platform for real-time visualization of diverse protein-DNA interactions. The present protocol utilizes the DNA curtain technique to investigate the biological role and molecular mechanism of Abo1, a Schizosaccharomyces pombe bromodomain-containing AAA+ ATPase.
