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基于半导体的下一代测序平台上非整倍体的植入前基因检测
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09:30 min
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August 17, 2022
DOI:
1Centre for Women, Children, and Reproduction,Guangxi Key Laboratory of Metabolic Diseases Research, 2Department of Biopharmaceutics, School of Laboratory Medicine and Biotechnology,Southern Medical University, 3Department of Obstetrics and Gynecology,Chinese PLA General Hospital, 4Clinical Laboratory,Northern Theater Air Force Hospital, 5Guangzhou Darui Reproduction Technology Co., Ltd.
Summary
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该协议介绍了在基于半导体的下一代测序平台上对非整倍性进行植入前基因检测所需的整体实验室内程序。在这里，我们以具有代表性的结果介绍了全基因组扩增、DNA片段选择、文库构建、模板制备和测序工作流程的详细步骤。
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Cite this Article
Xu, C., Wei, R., Lin, H., Deng, L., Wang, L., Li, D., Den, H., Qin, W., Wen, P., Liu, Y., Wu, Y., Ma, Q., Duan, J. Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform. J. Vis. Exp. (186), e63493, doi:10.3791/63493 (2022).
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