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一种重力喂养的经心灌注方法，用于小鼠中枢神经系统的组织学分析
Journal JoVE
Neurosciences
This content is Free Access.
Journal JoVE Neurosciences
A Gravity-Fed Transcardial Perfusion Method for Histologic Analysis of the Mouse Central Nervous System
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一种重力喂养的经心灌注方法，用于小鼠中枢神经系统的组织学分析

6,849 Views

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10:37 min

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January 21, 2022

DOI:

10.3791/63386-v

DOI:
10.3791/63386-v

•

10:37 min
January 21, 2022

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30 Views
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1Department of Biochemistry and Molecular Biology,State University of New York Upstate Medical University, 2Department of Neurology,State University of New York Upstate Medical University, 3Department of Neuroscience and Physiology,State University of New York Upstate Medical University

Summary

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提出了一种用于小鼠中枢神经系统组织学分析的方便的重力喂养灌注方法。磷酸化α突触核蛋白的免疫荧光检测在帕金森病的小鼠模型中得到证明。这项工作还全面描述了经心肌灌注，解剖，组织冷冻/包埋和冷冻切片步骤。

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Rana, A., Massa, P. T., Chen, X. J. A Gravity-Fed Transcardial Perfusion Method for Histologic Analysis of the Mouse Central Nervous System. J. Vis. Exp. (179), e63386, doi:10.3791/63386 (2022).

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