Journal
/
/
The Extraction of Liver Glycogen Molecules for Glycogen Structure Determination
Journal JoVE
Biochimie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biochimie
The Extraction of Liver Glycogen Molecules for Glycogen Structure Determination
DOI:

04:50 min

February 08, 2022

, , , ,

Chapitres

  • 00:00Introduction
  • 00:30Glycogen Extraction
  • 02:44Results: The Effect of Sucrose Concentration on the Particle Size of Glycogen
  • 04:25Conclusion

Summary

Traduction automatique

An optimal sucrose concentration was determined for the extraction of liver glycogen using sucrose density gradient centrifugation. The addition of a 10 min boiling step to inhibit glycogen-degrading enzymes proved beneficial.

Vidéos Connexes

Read Article