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Immunologie et infection

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流式细胞术分析鉴定鼠肺先天免疫细胞和适应性免疫细胞

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Immunologie et infection

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Journal JoVE Immunologie et infection

Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung

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流式细胞术分析鉴定鼠肺先天免疫细胞和适应性免疫细胞

5,506 Views

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09:57 min

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November 16, 2021

DOI:

10.3791/62985-v

DOI:

10.3791/62985-v

•

09:57 min

November 16, 2021

•

16 Views

Anthos Christofides^1,2, Carol Cao^1,2,3, Rinku Pal^1,2, Halil I. Aksoylar^1,2, Vassiliki A. Boussiotis^1,2

¹Division of Hematology-Oncology,Beth Israel Deaconess Medical Center, Harvard Medical School, ²Department of Medicine,Beth Israel Deaconess Medical Center, Harvard Medical School, ³Harvard College

Summary

在这项研究中，我们提出了一种有效且可重复的方案来分离小鼠呼吸系统的免疫群体。我们还提供了一种方法，使用基于9色的流式细胞术面板来鉴定驻留在健康小鼠肺部的所有先天性和适应性免疫细胞。

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