Optogenetic Phase Transition of TDP-43 in Spinal Motor Neurons of Zebrafish Larvae

Kazuhide Asakawa; Hiroshi Handa; Koichi Kawakami

doi:10.3791/62932

Journal

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Neurosciences

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Optogenetic Phase Transition of TDP-43 in Spinal Motor Neurons of Zebrafish Larvae

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Neurosciences

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Journal JoVE Neurosciences

Optogenetic Phase Transition of TDP-43 in Spinal Motor Neurons of Zebrafish Larvae

5,742 Views

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07:14 min

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February 25, 2022

DOI:

10.3791/62932-v

DOI:

10.3791/62932-v

•

07:14 min

February 25, 2022

•

3 Views

Kazuhide Asakawa¹, Hiroshi Handa¹, Koichi Kawakami^2,3

¹Department of Chemical Biology,Tokyo Medical University, ²Division of Molecular and Developmental Biology,National Institute of Genetics, ³Department of Genetics,Graduate University for Advanced Studies (SOKENDAI)

Summary

We describe a protocol to induce phase transition of TAR DNA-binding protein 43 (TDP-43) by light in the spinal motor neurons using zebrafish as a model.

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Optogenetic Phase Transition of TDP-43 in Spinal Motor Neurons of Zebrafish Larvae

Summary

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