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Immunology and Infection

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JoVE Journal Immunology and Infection
Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro
 

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro

Article DOI: 10.3791/62730-v • 06:12 min • March 7th, 2022
March 7th, 2022 •
1, 2, 1
1Graduate Institute of Immunology, National Taiwan University College of Medicine, 2Department of Pathology, Division of Microbiology and Immunology, University of Utah

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Here we provide a protocol for screening potential transcription factors involved in the development of dendritic cell (DC) using lentiviral transduction of shRNA to obtain stable knockdown cell lines for in vitro DC differentiation.

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Keywords: Dendritic Cell Development Short Hairpin RNA Gene Knockdown Hematopoietic Stem And Progenitor Cell Line In Vitro Transcription Factors FLT3 Ligand Beta Estradiol Lentiviral Transduction Puromycin LacZ Tcf4 Id2 Flow Cytometry
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