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Immunologie et infection

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Isolamento de células linfoides inatas uterinas para análise por citometria de fluxo

Journal JoVE
Immunologie et infection

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Journal JoVE Immunologie et infection

Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry

Isolamento de células linfoides inatas uterinas para análise por citometria de fluxo

3,860 Views

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09:02 min

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October 14, 2021

DOI:

10.3791/62670-v

DOI:

10.3791/62670-v

•

09:02 min

October 14, 2021

•

11 Views

Delphine M. Depierreux*^1,2, Emily Seshadri*^1,2, Evgeniya V. Shmeleva*^1,2, Jens Kieckbusch², Delia A. Hawkes, Francesco Colucci²

¹Department of Obstetrics and Gynaecology, National Institute for Health Research Cambridge Biomedical Research Centre,University of Cambridge School of Clinical Medicine, ²Centre for Trophoblast Research,University of Cambridge

Tags

Uterine Innate Lymphoid Cells Flow Cytometry Pregnant Non-pregnant Lymphocyte Isolation Cell Viability Fetus Removal Enzymatic Digestion EDTA PBS Cell Strainer

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