/

/

¹H R_{1 +} RNA 放松分散实验样品制备和设置的实用方面

Journal JoVE
Biochimie

This content is Free Access.

Journal JoVE Biochimie

Practical Aspects of Sample Preparation and Setup of ¹H R_1ρ Relaxation Dispersion Experiments of RNA

Please note that all translations are automatically generated. Click here for the English version.

¹H R_{1 +} RNA 放松分散实验样品制备和设置的实用方面

4,261 Views

•

08:17 min

•

July 09, 2021

DOI:

10.3791/62470-v

DOI:

10.3791/62470-v

•

08:17 min

July 09, 2021

•

1 Views

Hannes Feyrer¹, Judith Schlagnitweit¹, Katja Petzold¹

¹Department of Medical Biochemistry and Biophysics,Karolinska Institutet

Summary

我们提出了一个协议，测量微到毫秒的动态¹³^C/15N标签和未标记RNA与^1H R₁₊放松分散核磁共振（NMR）光谱。此协议的重点在于高纯度样品制备和 NMR 实验的设置。

Explore More Videos

RNA Sample Preparation NMR Setup Proton R1ρ Relaxation Dispersion Conformational Exchange In Vitro Transcription RNase H Cleavage HPLC Purification NMR Tube Cleaning D2O Addition

Read Article