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Microinjection of Corn Planthopper, Peregrinus maidis, Embryos for CRISPR/Cas9 Genome Editing
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Microinjection of Corn Planthopper, Peregrinus maidis, Embryos for CRISPR/Cas9 Genome Editing
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March 26, 2021
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Summary
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Herein are protocols for collecting and microinjecting precellular corn planthopper embryos for the purpose of modifying their genome via CRISPR/Cas9-based genome editing or for the addition of marked transposable elements through germline transformation.
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Klobasa, W., Chu, F., Huot, O., Grubbs, N., Rotenberg, D., Whitfield, A. E., Lorenzen, M. D. Microinjection of Corn Planthopper, Peregrinus maidis, Embryos for CRISPR/Cas9 Genome Editing. J. Vis. Exp. (169), e62417, doi:10.3791/62417 (2021).
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