Journal
/
生物工程
/
Microinjection of Corn Planthopper, Peregrinus maidis, Embryos for CRISPR/Cas9 Genome Editing
JoVE 杂志
生物工程
This content is Free Access.
JoVE 杂志 生物工程
Microinjection of Corn Planthopper, Peregrinus maidis, Embryos for CRISPR/Cas9 Genome Editing

Microinjection of Corn Planthopper, Peregrinus maidis, Embryos for CRISPR/Cas9 Genome Editing

DOI:
10.3791/62417-v

•

07:27 min

•

March 26, 2021

•

, , , , , ,
1Department of Entomology and Plant Pathology,North Carolina State University

Chapters

  • 00:04Introduction
  • 01:08Preparation of Agarose‐Based Egg‐Lay Chamber
  • 02:23Embryo Collection and Alignment
  • 02:59Microinjection and Post‐Injection Care
  • 03:48Incubation and Hatching of Embryos
  • 05:03Results: Microinjection of P. maidis Embryos for CRISPR/Cas9 Genome Editing and Collection
  • 06:32Conclusion

Summary

自动翻译

自动翻译

Herein are protocols for collecting and microinjecting precellular corn planthopper embryos for the purpose of modifying their genome via CRISPR/Cas9-based genome editing or for the addition of marked transposable elements through germline transformation.

Tags

Keywords: MicroinjectionCorn PlanthopperPeregrinus MaidisCRISPR/Cas9Genome EditingHemipteransTransgenicInsect EmbryosDesiccationThripsGene FunctionPest Control

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

check_url/cn/62417?article_type=v

Microinjection Wound Assay and In vivo Localization of Epidermal Wound Response Reporters in Drosophila Embryos.
check_url/cn/62417?article_type=v

A Full Skin Defect Model to Evaluate Vascularization of Biomaterials In Vivo
check_url/cn/62417?article_type=v

Ordering Single Cells and Single Embryos in 3D Confinement: A New Device for High Content Screening
check_url/cn/62417?article_type=v

Efficient Generation and Editing of Feeder-free IPSCs from Human Pancreatic Cells Using the CRISPR-Cas9 System
check_url/cn/62417?article_type=v

Production of Genetically Engineered Golden Syrian Hamsters by Pronuclear Injection of the CRISPR/Cas9 Complex
check_url/cn/62417?article_type=v

Microinjection of Western Corn Rootworm, Diabrotica virgifera virgifera, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing
check_url/cn/62417?article_type=v

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
check_url/cn/62417?article_type=v

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
check_url/cn/62417?article_type=v

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art
check_url/cn/62417?article_type=v

Prospecting Microbial Strains for Bioremediation and Probiotics Development for Metaorganism Research and Preservation

Read Article