Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Estimation of Plant Biomass Lignin Content using Thioglycolic Acid (TGA)
Chapitres
- 00:00Introduction
- 01:15Protocol: Phase I- Preparation of Plant Samples
- 02:34Protocol: Phase II- Preparation of Alcohol Insoluble Material (Water and Methanol Washes)
- 03:46Protocol: Phase III- Treatment of Pellet with TGA and Acid to Precipitate Lignin
- 06:22Protocol: Phase IV- Results (Ab280mm Reading)
- 07:01Representative Results
- 08:38Conclusion
Here, we present a modified TGA method for estimation of lignin content in herbaceous plant biomass. This method estimates the lignin content by forming specific thioether bonds with lignin and presents an advantage over the Klason method, as it requires a relatively small sample for lignin content estimation.
