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Sessiz İnsan Hematopoetik Kök Hücrelerini Korumak İçin Bir Kültür Yöntemi
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Bio-ingénierie
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Journal JoVE Bio-ingénierie
A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells
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Sessiz İnsan Hematopoetik Kök Hücrelerini Korumak İçin Bir Kültür Yöntemi

4,189 Views

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07:14 min

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May 17, 2021

DOI:

10.3791/61938-v

DOI:
10.3791/61938-v

•

07:14 min
May 17, 2021

•

12 Views
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1Department of Stem Cell Biology, Research Institute,National Center for Global Health and Medicine

Summary

Automatically generated

Bu protokol, bol miktarda yağ asidi, kolesterol, daha düşük sitokin konsantrasyonları ve hipoksi kullanarak kemik iliği mikroçevrimini taklit ederek sessiz insan hematopoetik kök hücrelerinin in vitro olarak korunmasını sağlar.

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Cite this Article

Kobayashi, H., Takubo, K. A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells. J. Vis. Exp. (171), e61938, doi:10.3791/61938 (2021).

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