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Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale
Journal JoVE
Bio-ingénierie
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Journal JoVE Bio-ingénierie
Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

DOI:
10.3791/61802-v

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08:49 min

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May 28, 2021

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, , , , ,
1Adolphe Merkle Institute,University of Fribourg, 2Dow Silicones Belgium SRL, 3Department of Biomedical and Specialist Surgical Sciences,University of Ferrara, 4Department of Animal Sciences, Plants for Human Health Institute,North Carolina State University

Chapitres

  • 00:04Introduction
  • 01:06Culture and Seeding of NHEKs
  • 05:02Cultivation at ALI
  • 05:47Results: Characterization of RhEs Cultivated From Neonatal NHEKs
  • 08:13Conclusion

Summary

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This protocol describes a straightforward method to cultivate three-dimensional reconstituted human epidermis in a reproducible and robust manner. Additionally, it characterizes the structure-function relationship of the epidermal barrier model. The biological responses of the reconstituted human epidermis upon proinflammatory stimuli are also presented.

Tags

3D Epidermal ModelReconstructed Human Epidermis (RHE)Normal Human Epidermal Keratinocytes (NHEKs)Cell CultureIn VitroLarge-scale CultivationCell SeedingCell PassagingTrypsinizationCell SuspensionSubmerged CultureAir-liquid Interface Culture

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