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Immunologie et infection
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Visualization of Pseudomonas aeruginosa within the Sputum of Cystic Fibrosis Patients
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Immunologie et infection
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Journal JoVE Immunologie et infection
Visualization of Pseudomonas aeruginosa within the Sputum of Cystic Fibrosis Patients

Visualization of Pseudomonas aeruginosa within the Sputum of Cystic Fibrosis Patients

2,859 Views

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07:25 min

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July 16, 2020

DOI:

10.3791/61631-v

DOI:
10.3791/61631-v

•

07:25 min
July 16, 2020

•

3 Views
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1Translational Medicine,Hospital for Sick Children, 2Center for Microbial Pathogenesis, Department of Pediatrics,University of Pittsburgh, 3Microbiology, Department of Pediatric Laboratory Medicine,Hospital for Sick Children, 4Infectious Diseases, Department of Pediatrics,Hospital for Sick Children

Summary

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This protocol provides methods for visualization of bacterial cells and polysaccharide synthesis locus (Psl) polysaccharide within the sputum of cystic fibrosis patients.

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Pseudomonas AeruginosaCystic FibrosisSputumVisualizationHydrogelExopolysaccharidePslBiofilmAntimicrobial SusceptibilityFixationAcrylamideAnaerobicSDSClearing

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Jackson, L., DePas, W., Morris, A. J., Guttman, K., Yau, Y. C. W., Waters, V. Visualization of Pseudomonas aeruginosa within the Sputum of Cystic Fibrosis Patients. J. Vis. Exp. (161), e61631, doi:10.3791/61631 (2020).

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