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脑毛细管中培养细胞细胞钙测量和钙成像研究
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Neurosciences
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Journal JoVE Neurosciences
Culture of Brain Capillary Pericytes for Cytosolic Calcium Measurements and Calcium Imaging Studies
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脑毛细管中培养细胞细胞钙测量和钙成像研究

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09:33 min

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May 27, 2020

DOI:

10.3791/61253-v

DOI:
10.3791/61253-v

•

09:33 min
May 27, 2020

•

5 Views
, ,
1Department of Pharmacy, The Faculty of Health and Medical Sciences,University of Copenhagen

Summary

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脑毛细管性信号是调节血脑屏障特性和血流的基本参与者。该协议描述了如何分离、培养脑毛细管腹膜，在细胞类型方面进行特征分析，并应用于用荧光探针研究细胞内钙信号。

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Cite this Article

Hørlyck, S., Helms, H. C. C., Brodin, B. Culture of Brain Capillary Pericytes for Cytosolic Calcium Measurements and Calcium Imaging Studies. J. Vis. Exp. (159), e61253, doi:10.3791/61253 (2020).

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