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Isolation of Cardiomyocytes from Fixed Hearts for Immunocytochemistry and Ploidy Analysis
Journal JoVE
Biologie
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Journal JoVE Biologie
Isolation of Cardiomyocytes from Fixed Hearts for Immunocytochemistry and Ploidy Analysis

Isolation of Cardiomyocytes from Fixed Hearts for Immunocytochemistry and Ploidy Analysis

DOI:
10.3791/60938-v

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08:41 min

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October 07, 2020

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, ,
1Department of Integrative Biology and Physiology,University of Minnesota, 2Lillehei Heart Institute, Department of Medicine,University of Minnesota, 3Stem Cell Institute,University of Minnesota

Chapitres

  • 00:04Introduction
  • 00:50Perfusion and Fixation of the Heart
  • 01:52Isolation of Fixed Cardiomyocytes
  • 03:17Image Quantification and Data Analysis
  • 04:53Data Analysis
  • 06:25Results: Segmentation and Classification of Isolated Cardiomyocytes
  • 08:19Conclusion

Summary

Traduction automatique

Traduction automatique

The goal of this work is to develop a method to reproducibly isolate cardiomyocytes from the adult heart and measure DNA content and nucleation.

Tags

CardiomyocytesIsolationImmunocytochemistryPloidy AnalysisFixed HeartsPerfusionCollagenaseDissociationFiltrationCentrifugation

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