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Isolation of Cardiomyocytes from Fixed Hearts for Immunocytochemistry and Ploidy Analysis
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Biologie
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JoVE Journal Biologie
Isolation of Cardiomyocytes from Fixed Hearts for Immunocytochemistry and Ploidy Analysis

Isolation of Cardiomyocytes from Fixed Hearts for Immunocytochemistry and Ploidy Analysis

6,572 Views

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08:41 min

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October 07, 2020

DOI:

10.3791/60938-v

DOI:
10.3791/60938-v

•

08:41 min
October 07, 2020

•

9 Views
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1Department of Integrative Biology and Physiology,University of Minnesota, 2Lillehei Heart Institute, Department of Medicine,University of Minnesota, 3Stem Cell Institute,University of Minnesota

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The goal of this work is to develop a method to reproducibly isolate cardiomyocytes from the adult heart and measure DNA content and nucleation.

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CardiomyocytesIsolationImmunocytochemistryPloidy AnalysisFixed HeartsPerfusionCollagenaseDissociationFiltrationCentrifugation

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Yücel, D., Solinsky, J., van Berlo, J. H. Isolation of Cardiomyocytes from Fixed Hearts for Immunocytochemistry and Ploidy Analysis. J. Vis. Exp. (164), e60938, doi:10.3791/60938 (2020).

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