Journal
/
Chimie
/
Mass Spectrometry-Guided Genome Mining as a Tool to Uncover Novel Natural Products
Journal JoVE
Chimie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Chimie
Mass Spectrometry-Guided Genome Mining as a Tool to Uncover Novel Natural Products

Mass Spectrometry-Guided Genome Mining as a Tool to Uncover Novel Natural Products

DOI:
10.3791/60825-v

•

11:13 min

•

March 12, 2020

•

, , ,
1Department of Organic Chemistry, Institute of Chemistry,University of Campinas (UNICAMP), 2Center for Natural and Human Sciences,Federal University of ABC (UFABC)

Chapitres

  • 00:04Introduction
  • 02:54Genome Mining for Biosynthetic Gene Clusters
  • 03:46Strain Cultivation
  • 04:51Acquiring Mass Spectra and Preparation for GNPS Analysis
  • 05:43GNPS Analysis
  • 08:33Results: Genome Sequence Analysis and Molecular Networking
  • 09:46Conclusion

Summary

Traduction automatique

Traduction automatique

A mass spectrometry-guided genome mining protocol is established and described here. It is based on genome sequence information and LC-MS/MS analysis and aims to facilitate identification of molecules from complex microbial and plant extracts.

Tags

Keywords: Mass SpectrometryGenome MiningNatural Product DiscoveryMolecular NetworkingWhole Genome SequencingGenotype-to-chemotypeBiosynthetic ClustersSecondary MetabolismGNPSStreptomycesFungiPlants

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/60825?article_type=v

Chemical Gardens as Flow-through Reactors Simulating Natural Hydrothermal Systems
check_url/fr/60825?article_type=v

A Direct, Early Stage Guanidinylation Protocol for the Synthesis of Complex Aminoguanidine-containing Natural Products
check_url/fr/60825?article_type=v

Laboratory Production of Biofuels and Biochemicals from a Rapeseed Oil through Catalytic Cracking Conversion
check_url/fr/60825?article_type=v

NMR Spectroscopy as a Robust Tool for the Rapid Evaluation of the Lipid Profile of Fish Oil Supplements
check_url/fr/60825?article_type=v

Coulomb Explosion Imaging as a Tool to Distinguish Between Stereoisomers
check_url/fr/60825?article_type=v

Electrochemical Impedance Spectroscopy as a Tool for Electrochemical Rate Constant Estimation
check_url/fr/60825?article_type=v

Color Spot Test As a Presumptive Tool for the Rapid Detection of Synthetic Cathinones
check_url/fr/60825?article_type=v

Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals
check_url/fr/60825?article_type=v

Employing Pressurized Hot Water Extraction (PHWE) to Explore Natural Products Chemistry in the Undergraduate Laboratory
check_url/fr/60825?article_type=v

A Two-Step Pyrolysis-Gas Chromatography Method with Mass Spectrometric Detection for Identification of Tattoo Ink Ingredients and Counterfeit Products

Read Article