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06:25 min
February 08, 2020
DOI:
10.3791/60731-v
我们描述了一种免疫荧光和定量PCR方法,用于昆虫组织中果子病毒的定位和定量。免疫荧光方案可用于共定病毒和载体蛋白。定量PCR协议可以扩展,以量化整个白蝇体内和受病毒感染的植物中的病毒。
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Ban, F., Yin, T., Guo, Q., Pan, L., Liu, Y., Wang, X. Localization and Quantification of Begomoviruses in Whitefly Tissues by Immunofluorescence and Quantitative PCR. J. Vis. Exp. (156), e60731, doi:10.3791/60731 (2020).
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