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PeptiQuick, ein Ein-Schritt-Einbindung von Membranproteinen in biotinylierte Peptidiscs für optimierte Proteinbindungstests
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Biochimie
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Journal JoVE Biochimie
PeptiQuick, a One-Step Incorporation of Membrane Proteins into Biotinylated Peptidiscs for Streamlined Protein Binding Assays
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PeptiQuick, ein Ein-Schritt-Einbindung von Membranproteinen in biotinylierte Peptidiscs für optimierte Proteinbindungstests

10,337 Views

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15:04 min

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November 02, 2019

DOI:

10.3791/60661-v

DOI:
10.3791/60661-v

•

15:04 min
November 02, 2019

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1 Views
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1Department of Biochemistry,University of British Columbia, 2School of Biochemistry,University of Bristol

Tags

Membrane ProteinsPeptidiscBiotinylated PeptidiscsProtein Binding AssaysDetergent-freeBio-layer InterferometryFhuAOuter Membrane VesiclesPeptiQuick ReconstitutionNickel-NTA IMACBiotinylated PeptideStreptavidin Coated Sensors

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Saville, J. W., Troman, L. A., Duong Van Hoa, F. PeptiQuick, a One-Step Incorporation of Membrane Proteins into Biotinylated Peptidiscs for Streamlined Protein Binding Assays. J. Vis. Exp. (153), e60661, doi:10.3791/60661 (2019).

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