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Immunology and Infection

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JoVE Journal Immunology and Infection
利用基于表观遗传学的定量PCR确定免疫细胞特性和纯度
 
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利用基于表观遗传学的定量PCR确定免疫细胞特性和纯度

Article DOI: 10.3791/60465-v • 08:02 min • February 19th, 2020
February 19th, 2020 •
1, 1, 1, 2, 1, 3, 3, 3, 1
1Cell Biology, Life Sciences Solutions, Thermo Fisher Scientific, 2California Polytechnic State University (Cal Poly), 3Epiontis GmbH, Precision for Medicine

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在这里，我们描述了一种通过使用定量PCR（qPCR）检测的表观遗传特征来确定免疫细胞特性和纯度的可靠方法。特定位点的脱甲基化作为特定细胞类型的唯一标识符，允许识别CD8+、调节或Th17 T细胞。

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免疫学和感染，第156期，qPCR，T细胞，身份，纯度，表观遗传学，DNA甲基化，双硫酸盐转化，细胞治疗
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