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基因敲击由CRISPR/Cas9和细胞排序在巨噬细胞和T细胞线
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Immunologie et infection
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Journal JoVE Immunologie et infection
Gene Knock-in by CRISPR/Cas9 and Cell Sorting in Macrophage and T Cell Lines
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基因敲击由CRISPR/Cas9和细胞排序在巨噬细胞和T细胞线

9,722 Views

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11:32 min

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November 13, 2021

DOI:

10.3791/62328-v

DOI:
10.3791/62328-v

•

11:32 min
November 13, 2021

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9 Views
, , , , , , , , ,
1The Laboratory of Genetic Regulators in the Immune System, School of Laboratory Medicine,Xinxiang Medical University, 2Centre d’Immunologie de Marseille-Luminy,Aix Marseille Université

Tags

CRISPR/Cas9Gene Knock-inMacrophageT Cell LinesROSA26 LocusSgRNA DesignHomologous RecombinationTargeting VectorFluorescent ReporterCell Sorting

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Zhang, L., Huang, R., Lu, L., Fu, R., Guo, G., Gu, Y., Liu, Z., He, L., Malissen, M., Liang, Y. Gene Knock-in by CRISPR/Cas9 and Cell Sorting in Macrophage and T Cell Lines. J. Vis. Exp. (177), e62328, doi:10.3791/62328 (2021).

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