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Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells
Journal JoVE
Recherche en cancérologie
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Journal JoVE Recherche en cancérologie
Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells

Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells

DOI:
10.3791/59997-v

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10:57 min

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October 24, 2019

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, , , , , ,
1Surgery Branch,National Cancer Institute, NIH, 2Center for Cell-Based Therapy,National Cancer Institute, NIH, 3Laboratory of Immunology, Institute for Frontier Life and Medical Sciences,Kyoto University

Chapitres

  • 00:04Titre
  • 00:58Passaging Human iPSCs on Mouse Embryonic Fibroblasts
  • 02:30Preparation of OP9/DLL1 Cells for Co-culture with hiPSCs
  • 03:51In Vitro Differentiation of hiPSCs into CD8αβ+
  • 08:38Results: hiPSC-derived Hematopoietic Progenitor Cells
  • 09:55Conclusion

Summary

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Traduction automatique

This article describes a method to generate functional tumor antigen-specific induced pluripotent stem cell-derived CD8αβ+ single positive T cells using OP9/DLL1 co-culture system.

Tags

Human Induced Pluripotent Stem CellsTumor Antigen-specific T CellsOP9-Delta-1 Co-cultureT Cell GenerationRegenerative MedicineCell-based TherapyNK CellsCell DifferentiationCell PassagingMouse Embryonic FibroblastsOP9 Media

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