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Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells
JoVE 杂志
癌症研究
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JoVE 杂志 癌症研究
Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells

Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells

9,926 Views

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10:57 min

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October 24, 2019

DOI:

10.3791/59997-v

DOI:
10.3791/59997-v

•

10:57 min
October 24, 2019

•

5 Views
, , , , , ,
1Surgery Branch,National Cancer Institute, NIH, 2Center for Cell-Based Therapy,National Cancer Institute, NIH, 3Laboratory of Immunology, Institute for Frontier Life and Medical Sciences,Kyoto University

Summary

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This article describes a method to generate functional tumor antigen-specific induced pluripotent stem cell-derived CD8αβ+ single positive T cells using OP9/DLL1 co-culture system.

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Human Induced Pluripotent Stem CellsTumor Antigen-specific T CellsOP9-Delta-1 Co-cultureT Cell GenerationRegenerative MedicineCell-based TherapyNK CellsCell DifferentiationCell PassagingMouse Embryonic FibroblastsOP9 Media

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Cite this Article

Good, M. L., Vizcardo, R., Maeda, T., Tamaoki, N., Malekzadeh, P., Kawamoto, H., Restifo, N. P. Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells. J. Vis. Exp. (152), e59997, doi:10.3791/59997 (2019).

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