Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Ex Vivo Oculomotor Slice Culture from Embryonic…
JoVE Journal
Neuroscience

Un abonnement à JoVE est nécessaire pour afficher ce contenu. Connectez-vous ou commencez votre essai gratuit.

JoVE Journal Neuroscience
Ex Vivo Oculomotor Slice Culture from Embryonic GFP-Expressing Mice for Time-Lapse Imaging of Oculomotor Nerve Outgrowth
 

Ex Vivo Oculomotor Slice Culture from Embryonic GFP-Expressing Mice for Time-Lapse Imaging of Oculomotor Nerve Outgrowth

Article DOI: 10.3791/59911-v • 06:04 min • July 16th, 2019
July 16th, 2019 •
1,2,3, 1,3, 1,3, 1,2,3,4,5,6
1Department of Ophthalmology, Boston Children's Hospital, 2Department of Ophthalmology, Harvard Medical School, 3F.M. Kirby Neurobiology Center, Boston Children's Hospital, 4Department of Neurology, Boston Children's Hospital, 5Department of Neurology, Harvard Medical School, 6Howard Hughes Medical Institute

Chapitres

résumé
Automatic Translation

An ex vivo slice assay allows oculomotor nerve outgrowth to be imaged in real time. Slices are generated by embedding E10.5 IslMN:GFP embryos in agarose, slicing on a vibratome, and growing in a stage-top incubator. The role of axon guidance pathways is assessed by adding inhibitors to the culture media.

Tags

Ex Vivo Oculomotor Slice Culture Embryonic GFP-expressing Mice Time-lapse Imaging Oculomotor Nerve Outgrowth Axon Guidance Pathways Nerve Trajectory Local Environments Axon Outgrowth Assessment Ocular Motor System Axon Guidance Study Working Quickly Pregnancy Confirmation Embryo Extraction Ice-cold HBSS Dissecting Scope Amniotic Sacs 12-well Plate
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter