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Preparation of Rat Sciatic Nerve for Ex Vivo Neurophysiology
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July 12, 2022
DOI:
1Department of Electrical and Electronic Engineering,Imperial College London, 2Centre for Bioinspired Technology,Imperial College London, 3Care Research and Technology (CR&T),Imperial College London and the University of Surrey, 4Dementia Institute (UK DRI), 5Department of Bioengineering,Imperial College London
Summary
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This protocol describes the preparation of rat whole sciatic nerve tissue for ex vivo electrophysiological stimulation and recording in an environmentally-regulated, two-compartment, perfused saline bath.
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Rapeaux, A., Syed, O., Cuttaz, E., Chapman, C. A. R., Green, R. A., Constandinou, T. G. Preparation of Rat Sciatic Nerve for Ex Vivo Neurophysiology. J. Vis. Exp. (185), e63838, doi:10.3791/63838 (2022).
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