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Experimental Analysis of Apoptotic Thymocyte Engulfment by Macrophages
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Experimental Analysis of Apoptotic Thymocyte Engulfment by Macrophages
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May 24, 2019
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Here, we present a protocol to prepare apoptotic thymocytes and peritoneal macrophages and analyze the efficiency of efferocytosis and the specific inhibitor-mediated blocking of apoptotic thymocytes engulfment. This protocol has a broad application in cell-mediated clearance of other particles including artificial beads and bacteria.
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Zhen, Y., Shao, W. Experimental Analysis of Apoptotic Thymocyte Engulfment by Macrophages. J. Vis. Exp. (147), e59731, doi:10.3791/59731 (2019).
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