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Experimental Analysis of Apoptotic Thymocyte Engulfment by Macrophages
Chapters
- 00:04Title
- 00:36Apoptotic Thymocyte Carboxyfluorescein Succinimidyl Ester (CFSE)-labeling
- 02:39Peritoneal Macrophage Preparation
- 03:53Peritoneal Macrophage: Apoptotic Thymocyte Co-culture
- 04:36Results: Representative Macrophage Phagocytosis and Efferocytosis Analyses
- 06:12Conclusion
Here, we present a protocol to prepare apoptotic thymocytes and peritoneal macrophages and analyze the efficiency of efferocytosis and the specific inhibitor-mediated blocking of apoptotic thymocytes engulfment. This protocol has a broad application in cell-mediated clearance of other particles including artificial beads and bacteria.
