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Generering av inducerade neurala stamceller från perifera mononukleära celler och differentiering mot dopaminerga neuron prekursorer för transplantations studier
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Biologie du développement
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Journal JoVE Biologie du développement
Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies
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Generering av inducerade neurala stamceller från perifera mononukleära celler och differentiering mot dopaminerga neuron prekursorer för transplantations studier

6,928 Views

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12:13 min

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July 11, 2019

DOI:

10.3791/59690-v

DOI:
10.3791/59690-v

•

12:13 min
July 11, 2019

•

1 Views
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1Cell Therapy Center, Beijing Institute of Geriatrics, Xuanwu Hospital, Capital Medical University, and Key Laboratory of Neurodegeneration,Ministry of Education, 2Center of Neural Injury and Repair,Beijing Institute for Brain Disorders

Tags

Induced Neural Stem CellsPeripheral Mononuclear CellsDopaminergic Neuron PrecursorsParkinson’s DiseaseAutologous Cell SourceProliferative CapacityRegion-specific Neural CellsNeurological DiseasesDensity Gradient MediumMononuclear CellsCell ExpansionCell FreezingCell Counting

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Cite this Article

Zheng, W., Chen, Z. Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies. J. Vis. Exp. (149), e59690, doi:10.3791/59690 (2019).

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