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Geração de células-tronco neurais induzidas de células mononucleares periféricas e diferenciação para precursores de neurónios dopaminérgicos para estudos de transplante
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Biologie du développement
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Journal JoVE Biologie du développement
Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies

Geração de células-tronco neurais induzidas de células mononucleares periféricas e diferenciação para precursores de neurónios dopaminérgicos para estudos de transplante

6,928 Views

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12:13 min

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July 11, 2019

DOI:

10.3791/59690-v

DOI:
10.3791/59690-v

•

12:13 min
July 11, 2019

•

1 Views
,
1Cell Therapy Center, Beijing Institute of Geriatrics, Xuanwu Hospital, Capital Medical University, and Key Laboratory of Neurodegeneration,Ministry of Education, 2Center of Neural Injury and Repair,Beijing Institute for Brain Disorders

Tags

Induced Neural Stem CellsPeripheral Mononuclear CellsDopaminergic Neuron PrecursorsParkinson’s DiseaseAutologous Cell SourceProliferative CapacityRegion-specific Neural CellsNeurological DiseasesDensity Gradient MediumMononuclear CellsCell ExpansionCell FreezingCell Counting

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Zheng, W., Chen, Z. Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies. J. Vis. Exp. (149), e59690, doi:10.3791/59690 (2019).

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