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Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging
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Chapitres
- 00:04Titre
- 00:51Micropattern Manufacturing Procedure
- 02:43Seeding Procedure
- 03:44Fixation
- 04:32Immunostaining
- 05:19Imaging and Image Analysis
- 07:55Results: Representative Micropattern Shape Analyses
- 09:25Conclusion
The method presented here uses micropatterning together with quantitative imaging to reveal spatial organization within mammalian cultures. The technique is easy to establish in a standard cell biology laboratory and offers a tractable system to study patterning in vitro.
