The method presented here uses micropatterning together with quantitative imaging to reveal spatial organization within mammalian cultures. The technique is easy to establish in a standard cell biology laboratory and offers a tractable system to study patterning in vitro.
Wisniewski, D., Lowell, S., Blin, G. Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging. J. Vis. Exp. (146), e59634, doi:10.3791/59634 (2019).