Journal
/
Biologie du développement
/
An In Vitro 3D Model and Computational Pipeline to Quantify the Vasculogenic Potential of iPSC-Derived Endothelial Progenitors
Journal JoVE
Biologie du développement
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie du développement
An In Vitro 3D Model and Computational Pipeline to Quantify the Vasculogenic Potential of iPSC-Derived Endothelial Progenitors

An In Vitro 3D Model and Computational Pipeline to Quantify the Vasculogenic Potential of iPSC-Derived Endothelial Progenitors

DOI:
10.3791/59342-v

•

06:36 min

•

May 13, 2019

•

,
1Department of Biomedical Engineering,University of Texas at Austin

Chapitres

  • 00:04Titre
  • 00:32Fluorescence-Activated Cell Sorting (FACS) of Endothelial Progenitors
  • 02:08Induced Pluripotent Stem Cell-Endothelial Progenitor (iPSC-EP)-Laden Collagen Hydrogel Encapsulation and Long-Term Culture
  • 03:29EP-Based Vascular Network Visualization
  • 05:22Results: Representative iPSC-EP Vascular Network Generation and Analysis
  • 06:06Conclusion

Summary

Traduction automatique

Traduction automatique

Endothelial progenitors derived from induced pluripotent stem cells (iPSC-EPs) have the potential to revolutionize cardiovascular disease treatments and to enable the creation of more faithful cardiovascular disease models. Herein, the encapsulation of iPSC-EPs in three-dimensional (3D) collagen microenvironments and a quantitative analysis of these cells’ vasculogenic potential are described.

Tags

Keywords: 3D ModelVasculogenesisIPSC-derived Endothelial ProgenitorsComputational PipelineCardiovascular Disease TherapyCell SortingCollagen Hydrogel EncapsulationCell CultureFACSROCK Inhibitor

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/59342?article_type=v

Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays
check_url/fr/59342?article_type=v

Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition
check_url/fr/59342?article_type=v

In Vitro and In Vivo Models to Study Corneal Endothelial-mesenchymal Transition
check_url/fr/59342?article_type=v

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
check_url/fr/59342?article_type=v

Generation of iPSC-derived Human Brain Organoids to Model Early Neurodevelopmental Disorders
check_url/fr/59342?article_type=v

The Aortic Ring Co-culture Assay: A Convenient Tool to Assess the Angiogenic Potential of Mesenchymal Stromal Cells In Vitro
check_url/fr/59342?article_type=v

Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea
check_url/fr/59342?article_type=v

Incorporating Pericytes into an Endothelial Cell Bead Sprouting Assay
check_url/fr/59342?article_type=v

Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro
check_url/fr/59342?article_type=v

Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton

Read Article