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An In Vitro 3D Model and Computational Pipeline to Quantify the Vasculogenic Potential of iPSC-Derived Endothelial Progenitors
Chapters
- 00:04Title
- 00:32Fluorescence-Activated Cell Sorting (FACS) of Endothelial Progenitors
- 02:08Induced Pluripotent Stem Cell-Endothelial Progenitor (iPSC-EP)-Laden Collagen Hydrogel Encapsulation and Long-Term Culture
- 03:29EP-Based Vascular Network Visualization
- 05:22Results: Representative iPSC-EP Vascular Network Generation and Analysis
- 06:06Conclusion
Endothelial progenitors derived from induced pluripotent stem cells (iPSC-EPs) have the potential to revolutionize cardiovascular disease treatments and to enable the creation of more faithful cardiovascular disease models. Herein, the encapsulation of iPSC-EPs in three-dimensional (3D) collagen microenvironments and a quantitative analysis of these cells’ vasculogenic potential are described.
