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An In Vitro 3D Model and Computational Pipeline to Quantify the Vasculogenic Potential of iPSC-Derived Endothelial Progenitors
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JoVE Revista Biología del desarrollo
An In Vitro 3D Model and Computational Pipeline to Quantify the Vasculogenic Potential of iPSC-Derived Endothelial Progenitors

An In Vitro 3D Model and Computational Pipeline to Quantify the Vasculogenic Potential of iPSC-Derived Endothelial Progenitors

DOI:
10.3791/59342-v

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06:36 min

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May 13, 2019

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,
1Department of Biomedical Engineering,University of Texas at Austin

Capítulos

  • 00:04Título
  • 00:32Fluorescence-Activated Cell Sorting (FACS) of Endothelial Progenitors
  • 02:08Induced Pluripotent Stem Cell-Endothelial Progenitor (iPSC-EP)-Laden Collagen Hydrogel Encapsulation and Long-Term Culture
  • 03:29EP-Based Vascular Network Visualization
  • 05:22Results: Representative iPSC-EP Vascular Network Generation and Analysis
  • 06:06Conclusion

Summary

Traducción Automática

Traducción Automática

Endothelial progenitors derived from induced pluripotent stem cells (iPSC-EPs) have the potential to revolutionize cardiovascular disease treatments and to enable the creation of more faithful cardiovascular disease models. Herein, the encapsulation of iPSC-EPs in three-dimensional (3D) collagen microenvironments and a quantitative analysis of these cells’ vasculogenic potential are described.

Tags

3D ModelVasculogenesisIPSC-derived Endothelial ProgenitorsComputational PipelineCardiovascular Disease TherapyCell SortingCollagen Hydrogel EncapsulationCell CultureFACSROCK Inhibitor

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