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Forberedelse og immunostai-Myelinating Organotypic lillehjernen skive kulturer
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Neurosciences
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Journal JoVE Neurosciences
Preparation and Immunostaining of Myelinating Organotypic Cerebellar Slice Cultures

Forberedelse og immunostai-Myelinating Organotypic lillehjernen skive kulturer

DOI:
10.3791/59163-v

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09:41 min

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March 20, 2019

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, , , ,
1Sorbonne Université, Inserm, CNRS, Institut du Cerveau et de la Moelle épinière, ICM-GH Pitié-Salpétrière

Chapitres

  • 00:04Titre
  • 00:36Cerebellum Dissection and Sections Preparation
  • 03:58Slices Culture and Demyelination
  • 04:55Immunohistochemistry
  • 08:15Results: Myelin Immunostaining in Organotypic Cerebellar Slices
  • 09:03Conclusion

Summary

Traduction automatique

Traduction automatique

Her presenterer vi en metode for å forberede organotypic skive kulturer fra musen lillehjernen og myelin-skjeden farging av immunhistokjemi egnet for å undersøke mekanismer for myelination og remyelination i det sentrale nervesystemet.

Tags

Keywords: Myelinating Organotypic Cerebellar Slice CulturesMyelinationRemyelinationCerebellumDissectionImmunostaining

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