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Uso da tecnologia de MHC única cadeia para investigar Co-Genealogia na ativação de células T humanas CD8 +
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1Department of Microbiology and Immunology, Yong Loo Lin School of Medicine,National University of Singapore, 2Immunology Programme, Life Sciences Institute,National University of Singapore, 3Emerging Infectious Diseases Program,Duke-NUS Graduate Medical School, 4Singapore Immunology Network,A*STAR, 5NUS Graduate School for Integrative Sciences and Engineering (NGS),National University of Singapore, 6Department of Immunology, Wright-Fleming Institute,Imperial College London
Chapitres
- 00:04Titre
- 01:19Plating CHO Cells as Antigen Presenting Cells
- 04:03T Cell Activation Assay
- 07:52CHO Cell MHC Class I Staining
- 09:06Results: Investigation of Co-agonism in Human CD8+ T Cell Activation
- 10:46Conclusion
Este protocolo descreve o uso da única cadeia de MHC classe I complexos para investigar interações moleculares na ativação de células T CD8 + humana: geração de engenharia apresentadoras de celulas que expressam cadeia única constrói, cultura de clone humano de células T CD8 + e experimentos de ativação de células T.
