Probiotic Studies in Neonatal Mice Using Gavage

Freddy Francis; Natallia Varankovich; Byron Brook; Nelly Amenyogbe; Rym Ben-Othman; Bing Cai; Danny Harbeson; Aaron  C. Liu; Ben Dai; Shelly McErlane; Kris Andrews; Tobias  R. Kollmann; Pinaki Panigrahi

doi:10.3791/59074

Journal

/

Immunologie et infection

/

Probiotic Studies in Neonatal Mice Using Gavage

Journal JoVE
Immunologie et infection
Author Produced

This content is Free Access.

Journal JoVE Immunologie et infection

Probiotic Studies in Neonatal Mice Using Gavage

DOI:

10.3791/59074-v

•

10:36 min

•

January 27, 2019

•

Freddy Francis, Natallia Varankovich, Byron Brook, Nelly Amenyogbe, Rym Ben-Othman, Bing Cai, Danny Harbeson, Aaron C. Liu, Ben Dai, Shelly McErlane, Kris Andrews, Tobias R. Kollmann*^1,2, Pinaki Panigrahi*³

¹Department of Experimental Medicine,University of British Columbia, ²Department of Pediatrics,University of British Columbia, ³Department of Epidemiology and Pediatrics,University of Nebraska Medical Centre, ⁴Animal Care Services,University of British Columbia

Chapitres

00:00Titre
00:04Intra-esophageal Gavage of Neonatal Mouse – Day of Life 2
05:19Collection of Intestinal Samples for Colonization Analysis
08:38Lactobacillus plantarum Colonization with Gavage Everyday or Every Other Day
09:03Lactobacillus plantarum Gavaged Mice Co-housed with Untreated Mice
09:28Conclusion

Summary

Traduction automatique

This study details the process of gavaging precise amounts of probiotics to neonatal mice. The experimental set-up was optimized to include but is not limited to probiotic dosing, methods of administration, and quantification of bacteria in the intestines.

Probiotic Studies in Neonatal Mice Using Gavage

•

•

•

Chapitres

Summary

Traduction automatique

Tags

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Using Luciferase to Image Bacterial Infections in Mice

Depletion and Reconstitution of Macrophages in Mice

Culturing Microglia from the Neonatal and Adult Central Nervous System

Non-Invasive Model of Neuropathogenic Escherichia coli Infection in the Neonatal Rat

Analyzing the Functions of Mast Cells In Vivo Using ‘Mast Cell Knock-in‘ Mice

Transformation of Probiotic Yeast and Their Recovery from Gastrointestinal Immune Tissues Following Oral Gavage in Mice

Noninvasive Sampling of Mucosal Lining Fluid for the Quantification of In Vivo Upper Airway Immune-mediator Levels

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay

Disclosing Hemolymph Collection and Inoculation of Metarhizium Blastospores into Rhipicephalus Microplus Ticks Towards Invertebrate Pathology Studies

An Ex vivo Assay to Study Candida albicans Hyphal Morphogenesis in the Gastrointestinal Tract

Read Article